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SERCA2 ATPase Antibody (10F0M9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15429

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15429-100ul
NBP3-15429-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10F0M9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 943-1042 of human SERCA2 ATPase (P16615). HFLILYVEPLPLIFQITPLNVTQWLMVLKISLPVILMDETLKFVARNYLEPGKECVQPATKSCSFSACTDGISWPFVLLIMPLVIWVYSTDTNFSDMFWS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SERCA2 ATPase Antibody (10F0M9)

Western Blot: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Western Blot: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Western Blot: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429] - Western blot analysis of extracts of various cell lines, using SERCA2 ATPase Rabbit mAb (NBP3-15429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Immunoprecipitation: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Immunoprecipitation: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SERCA2 ATPase antibody (NBP3-15429). Western blot was performed from the immunoprecipitate using SERCA2 ATPase antibody (NBP3-15429) at a dilition of 1:3000.

Applications for SERCA2 ATPase Antibody (10F0M9)

Application
Recommended Usage

Immunoprecipitation

1:1000 - 1:5000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SERCA2 ATPase

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Alternate Names

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2

Gene Symbol

ATP2A2

Additional SERCA2 ATPase Products

Product Documents for SERCA2 ATPase Antibody (10F0M9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SERCA2 ATPase Antibody (10F0M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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