SERCA2 ATPase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35164
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence within amino acids 1-80 of human SERCA2 ATPase (NP_733765.1).
Sequence:
MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEE
Sequence:
MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SERCA2 ATPase Antibody
Western Blot: SERCA2 ATPase Antibody [NBP3-35164] -
Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western Blot: SERCA2 ATPase Antibody [NBP3-35164] -
Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] -
Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using SERCA2 ATPase Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.Applications for SERCA2 ATPase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SERCA2 ATPase
Long Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Alternate Names
ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2
Gene Symbol
ATP2A2
Additional SERCA2 ATPase Products
Product Specific Notices for SERCA2 ATPase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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