Serotonin Antibody (YC5/45) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65037

Novus Biologicals, part of Bio-Techne
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NB100-65037SS
NB100-65037
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Key Product Details

Species Reactivity

Validated:

All Species

Cited:

Frog - Xenopus (African Clawed Frog), Primate - Macaca leonina (Northern Pig-Tailed Macaque)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # YC5/45

Format

BSA Free

Concentration

1.0 mg/ml

View all Serotonin Antibodies »

Product Specifications

Immunogen

Serotonin conjugated to Bovine Serum Albumin.

Reactivity Notes

This antibody detects serotonin in multiple species including human, mouse, rat, and other vertebrate and invertebrate animals.

Specificity

NB100-65037 recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin. Serotonin (also known as 5-hydroxytryptamine or 5-HT) is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for Serotonin Antibody (YC5/45) - BSA Free

Immunohistochemistry-Paraffin: Serotonin Antibody (YC5/45) - BSA Free [NB100-65037]

Immunohistochemistry-Paraffin: Serotonin Antibody (YC5/45) - BSA Free [NB100-65037]

Immunohistochemistry-Paraffin: Serotonin Antibody (YC5/45) [NB100-65037] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Serotonin antibody (clone YC5/45). The signal was developed using HRP-DAB method and the nuclei were counterstained using hematoxylin. This antibody generated a specific strong staining in serotonin positive neurons.

Applications for Serotonin Antibody (YC5/45) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 7781904)

Immunohistochemistry-Frozen

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serotonin

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20um) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. Useful for the diagnosis of carcinoid tumors and the investigation of the effects of drugs on the neurological system.

Alternate Names

5-HT, 5-hydroxytryptamne

Additional Serotonin Products

Product Documents for Serotonin Antibody (YC5/45) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Serotonin Antibody (YC5/45) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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