Serpin A1/alpha 1-Antitrypsin Antibody (2B12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47444

Novus Biologicals, part of Bio-Techne
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NBP1-47444
NBP1-47444-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Dot Blot, ELISA, Western Blot

Cited:

Cytometric Bead Assay Standard

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B12

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Serpin A1/alpha 1-Antitrypsin Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Serpin A1/alpha 1-Antitrypsin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin A1/alpha 1-Antitrypsin Antibody (2B12) - BSA Free

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12)BSA Free [NBP1-47444]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12)BSA Free [NBP1-47444]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12) [NBP1-47444] - Analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).

Applications for Serpin A1/alpha 1-Antitrypsin Antibody (2B12) - BSA Free

Application
Recommended Usage

Dot Blot

1:100 - 1:2000

ELISA

1:10000

Western Blot

1:500 - 1:2000
Application Notes
Dot Blot reactivity reported in scientific literature (PMID: 23028890).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin A1/alpha 1-Antitrypsin

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

UniProt

P01009

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Serpin A1/alpha 1-Antitrypsin Antibody (2B12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin A1/alpha 1-Antitrypsin Antibody (2B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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