Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47957

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9A1

Concentration

1 mg/ml

View all Serpin A1/alpha 1-Antitrypsin Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector

Specificity

This antibody is specific for Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Western blot analysis of extracts (10ug) from 7 Human tissue by using NBP1-47957 ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)
Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957]

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alpha 1 Antitrypsin antibody, and then analyzed by flow cytometry.
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Applications for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Serpin A1/alpha 1-Antitrypsin

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

UniProt

P01009

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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