Serpin A1/alpha 1-Antitrypsin Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7635
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Serpin A1/alpha 1-Antitrypsin [Human Plasma] (Uniprot: P01009)
Reactivity Notes
Cross reactivity against Serpin A1/alpha 1-Antitrypsin from other sources is unknown.
Localization
Secreted
Specificity
Cross reactivity against Serpin A1/alpha 1-Antitrypsin from other sources is unknown.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]
Scientific Data Images for Serpin A1/alpha 1-Antitrypsin Antibody [HRP]
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Western Blot of Serpin A1/alpha 1-Antitrypsin antibody [HRP]Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Serpin A1/alpha 1-Antitrypsin antibody at 1:3000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT.Block buffer overnight at 4C.Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Western Blot of Serpin A1/alpha 1-Antitrypsin antibody [HRP]. Lane 1: molecular weight. Lane 2: Goat-anti-Serpin A1/alpha 1-Antitrypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 ug per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST.Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in blocking buffer.Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C.Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
ELISA: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - ELISA Results of Serpin A1/alpha 1-Antitrypsin antibody [HRP]. Each well was coated in duplicate with 1.0 ug of Serpin A1/alpha 1-Antitrypsin. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer , Serpin A1/alpha 1-Antitrypsin antibody [HRP] and TMB substrate.
Applications for Serpin A1/alpha 1-Antitrypsin Antibody [HRP]
Application
Recommended Usage
ELISA
1:1000-5000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:500-1:2500
Western Blot
1:500-1:2500
Application Notes
This product is tested by ELISA and western blot. This product detects a single band of the expected apparent molecular weight (~47 kDa) and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Serpin A1/alpha 1-Antitrypsin
Alternate Names
A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor
Gene Symbol
SERPINA1
UniProt
Additional Serpin A1/alpha 1-Antitrypsin Products
Product Documents for Serpin A1/alpha 1-Antitrypsin Antibody [HRP]
Product Specific Notices for Serpin A1/alpha 1-Antitrypsin Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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