Serpin A1/alpha 1-Antitrypsin Antibody

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-78098]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-78098] - Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in buffer over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (shown as green) DyLight488 Donkey anti-Mouse IgG (shown as blue), and DyLight649 Donkey anti-Goat IgG (shown as red) at 1:10000 (in buffer 30 min RT).