Serpin A8/Angiotensinogen Antibody (9Q5D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33190

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 9Q5D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serpin A8/Angiotensinogen Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Serpin A8/Angiotensinogen (P01019).

Sequence:
MAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGML

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin A8/Angiotensinogen Antibody (9Q5D4)

Serpin A8/Angiotensinogen Antibody (9Q5D4)

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] -

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Serpin A8/Angiotensinogen Antibody (9Q5D4)

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] -

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of lysates from Mouse liver, using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for Serpin A8/Angiotensinogen Antibody (9Q5D4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Serpin A8/Angiotensinogen

Angiotensin II is encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Alternate Names

AGT, Angiotensin II, Angiotensinogen, Aogen, PAT

Gene Symbol

AGT

Additional Serpin A8/Angiotensinogen Products

Product Documents for Serpin A8/Angiotensinogen Antibody (9Q5D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin A8/Angiotensinogen Antibody (9Q5D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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