Serpin B5/Maspin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-97377

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

ICC/IF (Negative), Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Serpin B5/Maspin Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human MASPIN (within residues 100-200). [Swiss-Prot# P36952]

Localization

Secreted, extracellular space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin B5/Maspin Antibody - BSA Free

Knockdown Validated: Serpin B5/Maspin Antibody - BSA Free [NB110-97377]

Knockdown Validated: Serpin B5/Maspin Antibody - BSA Free [NB110-97377]

Knockdown Validated: Serpin B5/Maspin Antibody [NB110-97377] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NB110-97377) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Serpin B5/Maspin AntibodyBSA Free [NB110-97377]

Western Blot: Serpin B5/Maspin AntibodyBSA Free [NB110-97377]

Western Blot: MASPIN Antibody [NB110-97377] - Detection of Maspin in HeLa whole cell extracts using NB110-97377.

Applications for Serpin B5/Maspin Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5 ug/ml
Application Notes
This MASPIN antibody is useful for Western blot, where a doublet is seen at ~40 kDa. Does not work in Immunocytochemistry/Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin B5/Maspin

MASPIN (mammary serine protease inhibitor; SERPINB5 or PI5), is a member of SERPIN superfamily (serine protease inhibitor/non-inhibitor) which also includes certain other proteins such as plasminogen activator inhibitors 1/2 and alpha1-antitrypsin. MASPIN is an extracellular matrix tumor supressor which blocks the proliferative, invasive, and metastatic properties of breast as well as prostate cancers. Because it does not undergo S (stressed) to R (relaxed) conformational transition characteristic of active SERPINS, MASPIN does not exhibit serine protease inhibitory activity. MASPIN expression is down-regulated in breast, prostate, gastric and melanoma cancers, however, this protein is overexpressed in certain other malignancies such as pancreatic, gallbladder, colorectal, and thyroid cancers which reflects that MASPIN may play different activities in different cell types. These observations may be explained by MASPIN's distinct subcellular localization - cytoplasmic, nuclear or both cytoplasmic-nuclear expression; by interactions with extracellular matrix; its structure and epigenetic modifications. MASPIN is also expressed in corneal epithelial tissue, wherein it controls the migration/adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Alternate Names

Maspin, PI5

Gene Symbol

SERPINB5

Additional Serpin B5/Maspin Products

Product Documents for Serpin B5/Maspin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Serpin B5/Maspin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.