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Serpin C1/Antithrombin-III Antibody (2B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00000462-M02

Novus Biologicals, part of Bio-Techne
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H00000462-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B12

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

SERPINC1 (AAH22309.1, 1 a.a. ~ 259 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAEPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK

Specificity

SERPINC1 - serpin peptidase inhibitor, clade C (antithrombin), member 1 (2B12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Serpin C1/Antithrombin-III Antibody (2B12)

Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02]

Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02]

Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02] - Analysis of SERPINC1 expression in transfected 293T cell line by SERPINC1 monoclonal antibody (M02), clone 2B12.Lane 1: SERPINC1 transfected lysate(29.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02]

Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02]

Western Blot: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02] - SERPINC1 monoclonal antibody (M02), clone 2B12. Analysis of SERPINC1 expression in human kidney.
Sandwich ELISA: Serpin C1/Antithrombin-III Antibody (2B12) [H00000462-M02] - Detection limit for recombinant GST tagged SERPINC1 is 3 ng/ml as a capture antibody.

Applications for Serpin C1/Antithrombin-III Antibody (2B12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Serpin C1/Antithrombin-III

Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties

Alternate Names

Antithrombin-III, ATIII

Gene Symbol

SERPINC1

OMIM

107300 (Human)

Additional Serpin C1/Antithrombin-III Products

Product Documents for Serpin C1/Antithrombin-III Antibody (2B12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Serpin C1/Antithrombin-III Antibody (2B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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