Serpin E1/PAI-1 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19773CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-19773AF350

Alexa Fluor 405

NBP1-19773AF405

Alexa Fluor 488

NBP1-19773AF488

Alexa Fluor 532

NBP1-19773AF532

Alexa Fluor 594

NBP1-19773AF594

Alexa Fluor 647

NBP1-19773AF647

Alexa Fluor 700

NBP1-19773AF700

Alexa Fluor 750

NBP1-19773AF750

Biotin

NBP1-19773B

DyLight 350

NBP1-19773UV

DyLight 405

NBP1-19773V

DyLight 488

NBP1-19773G

DyLight 550

NBP1-19773R

DyLight 594

NBP1-19773DL594

DyLight 650

NBP1-19773C

DyLight 680

NBP1-19773FR

DyLight 755

NBP1-19773IR

FITC

NBP1-19773F

HRP

NBP1-19773H

Janelia Fluor 525

NBP1-19773JF525

Janelia Fluor 549

NBP1-19773JF549

Janelia Fluor 585

NBP1-19773JF585

Janelia Fluor 635

NBP1-19773JF635

Janelia Fluor 646

NBP1-19773JF646

Janelia Fluor 669

NBP1-19773JF669

mFluor Violet 450 SE

NBP1-19773MFV450

mFluor Violet 500 SE

NBP1-19773MFV500

mFluor Violet 610 SE

NBP1-19773MFV610

Forumulation

Catalog #

BSA Free

NBP1-19773

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serpin E1/PAI-1 Antibodies »

Product Summary for Serpin E1/PAI-1 Antibody [CoraFluor™ 1]

Immunogen

A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35386330).

Localization

Secreted.

Specificity

PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Serpin E1/PAI-1 Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Serpin E1/PAI-1 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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