Serpin F2/alpha 2-Antiplasmin Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89394

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serpin F2/alpha 2-Antiplasmin Antibodies »

Product Summary for Serpin F2/alpha 2-Antiplasmin Antibody (001)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Serpin F2/alpha 2-Antiplasmin (Accession#: NP_000925.2; Met 1-Lys 491) .

Specificity

No cross-reactivity in ELISA with: Human SERPINA1 Human SERPINA5 Mouse SERPIND1 Mouse SERPINF1 Mouse SERPINF2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Serpin F2/alpha 2-Antiplasmin Antibody (001)

Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394]

Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394]

Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394]

Immunoprecipitation: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394]

Immunoprecipitation: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394] - Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL anti-SERPINF2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SERPINF2 rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDaObserved band size: 66 kDa

Applications for Serpin F2/alpha 2-Antiplasmin Antibody (001)

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin F2/alpha 2-Antiplasmin

Identification in human milk of complexes of matriptase with ATIII, A1AT, or A2AP, provides evidence that the proteolytic activity of matriptase, from cells that express no or low levels of HAI-1, may be controlled by secreted serpins. Sequencing analysis revealed the presence of two alpha2-PI gene variations, both in the second half of exon 10: a frameshift mutation and a G to A transition at nucleotide 11337 in codon 407. Observational study of gene-disease association. (HuGE Navigator). Thrombin activatable fibrinolysis inhibitor and alpha(2)-antiplasmin are not markers for recanalization in patients with ischemic stroke treated with recombinant tissue-type plasminogen activator. Fibrinolysis is amplified by converting alpha-antiplasmin from a plasmin inhibitor to a substrate. Hydroxyethyl starch (HES) dilution enhances fibrinolysis by diminishing alpha2-antiplasmin-plasmin interactions. the Arg6Trp polymorphism may play a significant role in governing the long-term deposition/removal of intravascular fibrin. TAFIa, PAI-1 and alpha-antiplasmin: complementary roles in regulating lysis of thrombi and plasma clots. Alpha2-antiplasmin has an important role in acute pulmonary embolism. Multiple Lys residues within alpha 2-antiplasmin contribute, perhaps in a zipper-like fashion, to its binding to the in-tandem, multikringle array that configures the plasmin heavy chain. alpha2-antiplasmin induction inhibits E-cadherin processing mediated by the plasminogen activator/plasmin system, leading to suppression of progression of oral squamous cell carcinoma via upregulation of cell-cell adhesion

Alternate Names

alpha 2-Antiplasmin

Gene Symbol

SERPINF2

Additional Serpin F2/alpha 2-Antiplasmin Products

Product Documents for Serpin F2/alpha 2-Antiplasmin Antibody (001)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Serpin F2/alpha 2-Antiplasmin Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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