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Serum Response Factor SRF Antibody (1C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006722-M02

Novus Biologicals, part of Bio-Techne
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H00006722-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SRF (AAH48211, 406 a.a. ~ 508 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE

Specificity

SRF - serum response factor (c-fos serum response element-binding transcription factor)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Serum Response Factor SRF Antibody (1C8)

Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02]

Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02]

Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02] - SRF monoclonal antibody (M02), clone 1C8. Analysis of SRF expression in HeLa.
Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02]

Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02]

Western Blot: Serum Response Factor SRF Antibody (1C8) [H00006722-M02] - SRF monoclonal antibody (M02), clone 1C8 Analysis of SRF expression in Hela S3 NE.
Sandwich ELISA: Serum Response Factor SRF Antibody (1C8) [H00006722-M02] - Detection limit for recombinant GST tagged SRF is approximately 0.1ng/ml as a capture antibody.

Applications for Serum Response Factor SRF Antibody (1C8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Serum Response Factor SRF

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Long Name

Serum response factor

Alternate Names

SRF

Entrez Gene IDs

6722 (Human)

Gene Symbol

SRF

OMIM

600589 (Human)

Additional Serum Response Factor SRF Products

Product Documents for Serum Response Factor SRF Antibody (1C8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Serum Response Factor SRF Antibody (1C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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