Serum Response Factor SRF Antibody (PCRP-SRF-1F7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14188
![Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) - Azide and BSA Free [NBP3-14188]](https://resources.bio-techne.com/images/products/Serum-Response-Factor-SRF-Antibody-PCRP-SRF-1F7-Azide-and-BSA-Free-Protein-Array-NBP3-14188-img0001.jpg "Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) - Azide and BSA Free [NBP3-14188]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-SRF-1F7
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
Recombinant full-length human Serum Response Factor SRF protein (Uniprot: P11831)
Localization
Nucleus, Nucleoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13785).
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 40-67kDa
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 40-67kDa
Scientific Data Images
Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) - Azide and BSA Free [NBP3-14188]
Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) - Azide and BSA Free [NBP3-14188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Serum Response Factor SRF Antibody (PCRP-SRF-1F7).Applications
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Serum Response Factor SRF
Long Name
Serum response factor
Alternate Names
SRF
Gene Symbol
SRF
Additional Serum Response Factor SRF Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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