SESN2 Antibody (3B8)
Novus Biologicals, part of Bio-Techne | Catalog # H00083667-M03
Key Product Details
Species Reactivity
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3B8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SESN2 (AAH13304.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT
Reactivity Notes
Other species not tested.
Specificity
SESN2 - sestrin 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for SESN2 Antibody (3B8)
Western Blot: SESN2 Antibody (3B8) [H00083667-M03]
Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8. Lane 1: SESN2 transfected lysatE (54.5 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03]
Immunocytochemistry/Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SES2 on HeLa cell. Antibody concentration 10 ug/mlImmunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03]
Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/mlApplications for SESN2 Antibody (3B8)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SESN2
Alternate Names
DKFZp761M0212, DKFZp761M02121, Hi95, HI95sestrin-2, SES2, SEST2hypoxia induced gene 95, sestrin 2
Entrez Gene IDs
83667 (Human)
Gene Symbol
SESN2
OMIM
607767 (Human)
UniProt
Additional SESN2 Products
Product Documents for SESN2 Antibody (3B8)
Product Specific Notices for SESN2 Antibody (3B8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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