SET Antibody (8E8M9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16775
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8E8M9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-290 of human SET (Q01105). ESFFTWFTDHSDAGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEGEEDDDDDEEEEGLEDIDEEGDEDEGEEDEDDDEGEEGEEDEGEDD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SET Antibody (8E8M9)
Western Blot: SET Antibody (8E8M9) [NBP3-16775]
Western Blot: SET Antibody (8E8M9) [NBP3-16775] - Western blot analysis of extracts of various cell lines, using SET Rabbit mAb (NBP3-16775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: SET Antibody (8E8M9) [NBP3-16775]
Immunocytochemistry/Immunofluorescence: SET Antibody (8E8M9) [NBP3-16775] - Immunofluorescence analysis of U-2 OS cells using SET Rabbit mAb (NBP3-16775) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: SET Antibody (8E8M9) [NBP3-16775]
Immunohistochemistry-Paraffin: SET Antibody (8E8M9) [NBP3-16775] - Immunohistochemistry of paraffin-embedded mouse spleen using SET Rabbit mAb (NBP3-16775) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for SET Antibody (8E8M9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SET
Alternate Names
2PP2A, HLA-DR-associated protein II, I2PP2A, I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPIITAF-I, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, protein SET, SET nuclear oncogene, SET translocation (myeloid leukemia-associated), TAF-IBETA, Template-activating factor I, Template-Activating Factor-I, chromatin remodelling factor
Gene Symbol
SET
Additional SET Products
Product Documents for SET Antibody (8E8M9)
Product Specific Notices for SET Antibody (8E8M9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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