SET Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-817

Novus Biologicals, part of Bio-Techne
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NB100-817
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SET Antibodies »

Product Specifications

Immunogen

Peptide with sequence SAPAAKVSKKELNS-C corresponding to N-Terminus according to NP_003002.2.

Specificity

This antibody is expected to recognize isoform 2 (NP_003002.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SET Antibody

Western Blot: SET Antibody [NB100-817]

Western Blot: SET Antibody [NB100-817]

Western Blot: SET Antibody [NB100-817] - Staining (0.01 ug/ml) of Daudi (A) and Molt4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: SET Antibody [NB100-817]

Western Blot: SET Antibody [NB100-817]

Western Blot: SET Antibody [NB100-817] - Staining (03ug/ml) of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SET Antibody

Immunohistochemistry-Paraffin: SET Antibody [NB100-817] -

Immunohistochemistry-Paraffin: SET Antibody [NB100-817] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
View 1 more images

Applications for SET Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

7 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in lysates of human kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1 kDa band according to NP_003002.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SET

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Alternate Names

2PP2A, HLA-DR-associated protein II, I2PP2A, I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPIITAF-I, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, protein SET, SET nuclear oncogene, SET translocation (myeloid leukemia-associated), TAF-IBETA, Template-activating factor I, Template-Activating Factor-I, chromatin remodelling factor

Entrez Gene IDs

6418 (Human)

Gene Symbol

SET

UniProt

AAC50460 (Human)

Additional SET Products

Product Documents for SET Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SET Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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