Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SET1B Antibodies »

Product Summary for SET1B Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1400 and 1450 of human SET domain containing 1B using the numbering given in entry NP_055863.1 (GeneID 23067).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SET1B Antibody

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hSET1B used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: SET1B Antibody [NBP1-28685]

Immunoprecipitation: SET1B Antibody [NBP1-28685]

Immunoprecipitation: SET1B Antibody [NBP1-28685] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SET1B antibody NBP1-28685 used for IP at 10 ug/mg lysate. SET1B was also immunoprecipitated by rabbit anti-SET1B antibody NBP1-28684, which recognizes an upstream epitope. For blotting immunoprecipitated SET1B, NBP1-28684 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SET1B Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SET1B

hSET1B is a histone methyltransferase that associates in a complex with the non-catalytic subunits CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. These five non-catalytic subunits are also part of the Set1A complex. The SET1B complex has been shown to catalyze the tri-methylation of 'Lys-4' on histone H3. SET1B has been shown to localize to sites independent of SET1A complexes indicating non-redundant functions between SET1B and SET1A complexes. hSET1B include SET domain-containing protein 1B, lysine N-methyltransferase 2G, SETD1B, KMT2G, SET1B, and KIAA1076.

Alternate Names

EC 2.1.1, FLJ20803, histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076EC 2.1.1.43, KMT2GSET1B, Lysine N-methyltransferase 2G, SET domain containing 1B, SET domain-containing protein 1B, Set1B

Entrez Gene IDs

23067 (Human)

Gene Symbol

SETD1B

UniProt

NP_055863.1 (Human)

Additional SET1B Products

Product Documents for SET1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SET1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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