Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SET1B Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1400 and 1450 of human SET domain containing 1B using the numbering given in entry NP_055863.1 (GeneID 23067).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SET1B Antibody

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28685] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hSET1B used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: SET1B Antibody [NBP1-28685]

Immunoprecipitation: SET1B Antibody [NBP1-28685]

Immunoprecipitation: SET1B Antibody [NBP1-28685] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SET1B antibody NBP1-28685 used for IP at 10 ug/mg lysate. SET1B was also immunoprecipitated by rabbit anti-SET1B antibody NBP1-28684, which recognizes an upstream epitope. For blotting immunoprecipitated SET1B, NBP1-28684 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SET1B Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SET1B

hSET1B is a histone methyltransferase that associates in a complex with the non-catalytic subunits CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. These five non-catalytic subunits are also part of the Set1A complex. The SET1B complex has been shown to catalyze the tri-methylation of 'Lys-4' on histone H3. SET1B has been shown to localize to sites independent of SET1A complexes indicating non-redundant functions between SET1B and SET1A complexes. hSET1B include SET domain-containing protein 1B, lysine N-methyltransferase 2G, SETD1B, KMT2G, SET1B, and KIAA1076.

Alternate Names

EC 2.1.1, FLJ20803, histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076EC 2.1.1.43, KMT2GSET1B, Lysine N-methyltransferase 2G, SET domain containing 1B, SET domain-containing protein 1B, Set1B

Entrez Gene IDs

23067 (Human)

Gene Symbol

SETD1B

UniProt

NP_055863.1 (Human)

Additional SET1B Products

Product Documents for SET1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SET1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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