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SET1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18682

Novus Biologicals, part of Bio-Techne
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NBP3-18682-100ul
NBP3-18682-25ul

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for SET1B Antibody

Immunogen

Polyclonal antibody raised in rabbit against mouse SET1B (Set domain containing 1B), using a 3 KLH-conjugated synthetic peptides containing sequences from the central part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for SET1B Antibody

Western Blot: SET1B Antibody [NBP3-18682]

Western Blot: SET1B Antibody [NBP3-18682]

Western Blot: SET1B Antibody [NBP3-18682] - Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse SET1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
ELISA: SET1B Antibody [NBP3-18682]

ELISA: SET1B Antibody [NBP3-18682]

ELISA: SET1B Antibody [NBP3-18682] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse SET1B . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,400.

Applications for SET1B Antibody

Application
Recommended Usage

ELISA

1:100-1:500

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SET1B

hSET1B is a histone methyltransferase that associates in a complex with the non-catalytic subunits CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. These five non-catalytic subunits are also part of the Set1A complex. The SET1B complex has been shown to catalyze the tri-methylation of 'Lys-4' on histone H3. SET1B has been shown to localize to sites independent of SET1A complexes indicating non-redundant functions between SET1B and SET1A complexes. hSET1B include SET domain-containing protein 1B, lysine N-methyltransferase 2G, SETD1B, KMT2G, SET1B, and KIAA1076.

Alternate Names

EC 2.1.1, FLJ20803, histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076EC 2.1.1.43, KMT2GSET1B, Lysine N-methyltransferase 2G, SET domain containing 1B, SET domain-containing protein 1B, Set1B

Gene Symbol

SETD1B

UniProt

Additional SET1B Products

Product Documents for SET1B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SET1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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