Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7B7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SETD1A Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-320 of human SETD1A(NP_055527) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

185.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SETD1A Antibody (OTI7B7) - Azide and BSA Free

Western Blot: SETD1A Antibody (OTI7B7)Azide and BSA Free [NBP2-74144]

Western Blot: SETD1A Antibody (OTI7B7)Azide and BSA Free [NBP2-74144]

Western Blot: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD1A.
Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144]

Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144]

Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144]

Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144]

Immunohistochemistry: SETD1A Antibody (OTI7B7) - Azide and BSA Free [NBP2-74144] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for SETD1A Antibody (OTI7B7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETD1A

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]

Alternate Names

histone-lysine N-methyltransferase SETD1A, hSET1A, KIAA0339EC 2.1.1.43, KMT2FSET1, Lysine N-methyltransferase 2F, SET domain containing 1A, SET domain-containing protein 1A, Set1, Set1/Ash2 histone methyltransferase complex subunit SET1, SET1A

Gene Symbol

SETD1A

Additional SETD1A Products

Product Documents for SETD1A Antibody (OTI7B7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SETD1A Antibody (OTI7B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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