SETD7/9 Antibody (19)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06624

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 19

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SETD7/9 Antibodies »

Product Summary for SETD7/9 Antibody (19)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SETD7/9 (Accession#: NP_085151.1; Asp2-Lys366).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SETD7/9 Antibody (19)

Western Blot: SETD7/9 Antibody (19) [NBP3-06624]

Western Blot: SETD7/9 Antibody (19) [NBP3-06624]

Western Blot: SETD7/9 Antibody (19) [NBP3-06624] - NBP3-06624 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: NIH3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique and performed under reducing conditions.

Applications for SETD7/9 Antibody (19)

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD7/9

Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human Set7/9 is a 336 amino acid protein.

Alternate Names

EC 2.1.1.43, H3-K4-HMTase SETD7, histone H3-lysine 4-specific methyltransferase, histone-lysine N-methyltransferase SETD7, KIAA1717SET domain-containing protein 7, KMT7FLJ21193, Lysine N-methyltransferase 7, SET domain containing (lysine methyltransferase) 7, SET7/9Histone H3-K4 methyltransferase SETD7, SET7Set9, SET9

Gene Symbol

SETD7

Additional SETD7/9 Products

Product Documents for SETD7/9 Antibody (19)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SETD7/9 Antibody (19)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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