SETD7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93288

Novus Biologicals, part of Bio-Techne
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NB100-93288
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SETD7 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 316 and 366 of human SET domain containing (lysine methyltransferase) 7 using the numbering given in entry NP_085151.1 (GeneID 80854).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SETD7 Antibody

Western Blot: SETD7 Antibody [NB100-93288]

Western Blot: SETD7 Antibody [NB100-93288]

Western Blot: SETD7 Antibody [NB100-93288] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93288 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: SETD7 Antibody [NB100-93288]

Immunohistochemistry-Paraffin: SETD7 Antibody [NB100-93288]

Immunohistochemistry-Paraffin: SETD7 Antibody [NB100-93288] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SET7 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Applications for SETD7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SETD7

SET7 is a member of the SET (SuVar3-9, enhancer of Zeste, Trithorax) domain-containing protein family. SET7 functions as a histone methyltransferase that specifically monomethylates lysine-4 of histone H3. SET7 has also been observed to monomethylate non-histone proteins such as p53, TAF10, and the estrogen receptor. The methylating activity of SET7 is essential to cell cycle progression and genomic stability.

Long Name

Histone-lysine N-methyltransferase SETD7

Alternate Names

EC 2.1.1.364, H3-K4-HMTase SETD7, KIAA1717, KMT7, SET7, SET7/9, SET9

Entrez Gene IDs

80854 (Human); 73251 (Mouse)

Gene Symbol

SETD7

UniProt

Q8WTS6 (Human)

Additional SETD7 Products

Product Documents for SETD7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SETD7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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