SETD8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99864
![Western Blot: SETD8 Antibody [NBP2-99864]](https://resources.bio-techne.com/images/products/SETD8-Antibody-Western-Blot-NBP2-99864-img0003.jpg "Western Blot: SETD8 Antibody [NBP2-99864]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SETD8 Antibody
Immunogen
Produced in rabbits immunized with purified, recombinant Human SETD8 (Accession#: NP_065115.3; Lys195-His352)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SETD8 Antibody
Western Blot: SETD8 Antibody [NBP2-99864]
Western Blot: SETD8 Antibody [NBP2-99864] - Anti-SETD8 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HCT-116 Whole Cell Lysate. Lane C: U87-MG Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa![Immunoprecipitation: SETD8 Antibody [NBP2-99864]](https://resources.bio-techne.com/images/products/SETD8-Antibody-Immunoprecipitation-NBP2-99864-img0004.jpg "Immunoprecipitation: SETD8 Antibody [NBP2-99864]")
Immunoprecipitation: SETD8 Antibody [NBP2-99864]
Immunoprecipitation: SETD8 Antibody [NBP2-99864] - SETD8 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-SETD8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-SETD8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDaApplications for SETD8 Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
5-10 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SETD8
Long Name
N-lysine methyltransferase KMT5A
Alternate Names
H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8
Gene Symbol
KMT5A
Additional SETD8 Products
Product Documents for SETD8 Antibody
Product Specific Notices for SETD8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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