Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SETDB1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1125 and 1175 of human SET Domain, Bifurcated 1 using the numbering given in entry NP_036564.2 (GeneID 9869).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETDB1 Antibody - BSA Free

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - Detection of human SETDB1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SETDB1 antibody NB100-79776 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - HeLa whole cell lysate using NB100-79776. Rabbit anti-SETDB1 antibodies BL538 and NB100-79776 were used at 1 mcg/mg lysate for IP (B).
Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SetDB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SETDB1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. [supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Entrez Gene IDs

9869 (Human)

Gene Symbol

SETDB1

UniProt

Q15047 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SETDB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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