SETDB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1137

Novus Biologicals, part of Bio-Techne
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NB100-1137
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Peptide ELISA, Western Blot

Cited:

Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SETDB2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence GEKNGDAKTFWME-C corresponding to N-Terminus according to NP_114121.2, NP_001153780.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.
Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.
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Applications for SETDB2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/mL
Application Notes
Approx 85 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9 kDa according to NP_114121.2). Not suitable on mouse heart and rat heart lysates. This SETDB2 antibody is validated for WB, CHIP, ICC/IF IP from verified customer reviews.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4 using NB100-1137 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETDB2

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity.

Alternate Names

C13orf4, chromosome 13 open reading frame 4, chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8 protein, CLLD8DKFZp761J1217, DKFZp586I0123, EC 2.1.1.43, histone-lysine N-methyltransferase SETDB2, KMT1FCLLL8, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SET domain, bifurcated 2

Entrez Gene IDs

83852 (Human)

Gene Symbol

SETDB2

UniProt

NP_114121. (Human)

Additional SETDB2 Products

Product Documents for SETDB2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SETDB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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