SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07806

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NR5A1/3397

Concentration

0.2 mg/ml

View all SF-1/NR5A1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa220-461) of human SF-1/NR5A1/Steroidogenic Factor 1 protein (Uniprot: Q13285)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08624)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397)

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806] - Formalin-fixed, paraffin-embedded human thymus stained with SF-1/NR5A1/Steroidogenic Factor 1 Mouse Monoclonal Antibody (NR5A1/3397).
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806] - Formalin-fixed, paraffin-embedded human testis stained with SF-1/NR5A1/Steroidogenic Factor 1 Mouse Monoclonal Antibody (NR5A1/3397).
Protein Array: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Protein Array: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806]

Protein Array: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397) [NBP3-07806] - Analysis of Protein Array containing >19,000 full-length human proteins using SF-1/NR5A1/Steroidogenic Factor 1 Mouse Monoclonal Antibody (NR5A1/3397).

Applications for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SF-1/NR5A1

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Long Name

Steroidogenic Factor 1

Alternate Names

AD4BP, FTZF1, NR5A1, SF1

Gene Symbol

NR5A1

UniProt

Q13285

Additional SF-1/NR5A1 Products

Product Documents for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/3397)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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