SF2 Antibody (3J5Q9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16329
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3J5Q9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SF2 Antibody (3J5Q9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SF2 (NP_008855.1). GGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SF2 Antibody (3J5Q9)
Western Blot: SF2 Antibody (3J5Q9) [NBP3-16329]
Western Blot: SF2 Antibody (8L3T3) [NBP3-16329] - Western blot analysis of extracts of various cell lines, using SRSF1/SF2 Rabbit mAb (NBP3-16329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]
Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded mouse brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]
Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded rat brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for SF2 Antibody (3J5Q9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SF2
Alternate Names
Alternative-splicing factor 1, ASFpre-mRNA-splicing factor SF2, P33 subunit, MGC5228, serine/arginine-rich splicing factor 1, SF2FLJ53078, SF2p33, SFRS1splicing factor 2, Splicing factor, arginine/serine-rich 1ASF-1, SR splicing factor 1, SRp30a
Gene Symbol
SRSF1
Additional SF2 Products
Product Documents for SF2 Antibody (3J5Q9)
Product Specific Notices for SF2 Antibody (3J5Q9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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