SF3B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79848

Novus Biologicals, part of Bio-Techne
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NB100-79848
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SF3B2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 825 and 872 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF3B2 Antibody

Western Blot: SF3B2 Antibody [NB100-79848]

Western Blot: SF3B2 Antibody [NB100-79848]

Western Blot: SF3B2 Antibody [NB100-79848] - Detection of Human SF3b145/SAP145 from HeLa whole cell lysate (5, 15, 50 microgram) and 293T (50 microgram) using NB100-79848. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody NB100-79847.
Immunohistochemistry-Paraffin: SF3B2 Antibody [NB100-79848]

Immunohistochemistry-Paraffin: SF3B2 Antibody [NB100-79848]

Immunohistochemistry-Paraffin: SF3B2 Antibody [NB100-79848] - Section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for SF3B2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-79848 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SF3B2

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Alternate Names

Cus1, pre-mRNA splicing factor SF3b 145 kDa subunit, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SAP145SAP 145, SF3b1, SF3B145, SF3b150, spliceosome associated protein 145, Spliceosome-associated protein 145, splicing factor 3B subunit 2, splicing factor 3b, subunit 2, 145kD, splicing factor 3b, subunit 2, 145kDa

Entrez Gene IDs

10992 (Human)

Gene Symbol

SF3B2

UniProt

Q13435 (Human)

Additional SF3B2 Products

Product Documents for SF3B2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF3B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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