Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 3A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SF3B4 Antibodies »

Product Specifications

Immunogen

Full length recombinant human SF3B4 expressed in and purified from E. coli. [UniProt# Q15427]

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for SF3B4 Antibody (3A1) [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This SF3B4 (3A1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 49 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SF3B4

SF3B4 (splicing factor 3B subunit 4, also known as SF3b50 or SAP49), is a ubiquitously expressed nuclear protein which forms a subunit of splicing factor SF3B (SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125) required for 'A' complex assembly formed by the stable binding of U2 snRNP to pre-mRNA's BPS (branchpoint sequence). SF3B4 interacts directly with SF3B2 and also forms a component of U11/U12 snRNPs that are a part of the U12-type spliceosome. SF3B4 has also been suggested to implicate in 'E' complex assembly and is also found in 'B' and 'C' complexes. SF3B4 is also associated with minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Defects in SF3B4 have been linked to AFD1 (acrofacial dysostosis type 1) which is a form of acrofacial dysostosis, a group of disorders characterized by malformation of the craniofacial skeleton and limbs.

Alternate Names

Hsh49, pre-mRNA splicing factor SF3b 49 kDa subunit, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49MGC10828, SF3B49, SF3b50, spliceosomal protein, spliceosome-associated protein (U2 snRNP), Spliceosome-associated protein 49, splicing factor 3B subunit 4, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa

Gene Symbol

SF3B4

Additional SF3B4 Products

Product Documents for SF3B4 Antibody (3A1) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SF3B4 Antibody (3A1) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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