SF3B4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1076

Novus Biologicals, part of Bio-Techne
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NB100-1076
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SF3B4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence AAGPISERNQDAT-C corresponding to N-Terminus according to NP_005841.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SF3B4 Antibody

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076] - Western blot analysis of SF3B4 in Hela lysate using NB100-1076 at 0.1 ug/ml.
Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076] - staining (0.1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for SF3B4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human HeLa lysates (calculated MW of 44.4 kDa band according to NP005841.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SF3B4

The SF3B4 gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

Alternate Names

Hsh49, pre-mRNA splicing factor SF3b 49 kDa subunit, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49MGC10828, SF3B49, SF3b50, spliceosomal protein, spliceosome-associated protein (U2 snRNP), Spliceosome-associated protein 49, splicing factor 3B subunit 4, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa

Entrez Gene IDs

10262 (Human); 107701 (Mouse)

Gene Symbol

SF3B4

UniProt

NP_005841. (Human)

Additional SF3B4 Products

Product Documents for SF3B4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF3B4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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