SF3B4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20326

Novus Biologicals, part of Bio-Techne
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NBP2-20326
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%), Rhesus Macaque (100%), Zebrafish (96%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SF3B4 Antibodies »

Product Summary for SF3B4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF3B4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (88%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SF3B4 Antibody

Western Blot: SF3B4 Antibody [NBP2-20326]

Western Blot: SF3B4 Antibody [NBP2-20326]

Western Blot: SF3B4 Antibody [NBP2-20326] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: SF3B4 Antibody [NBP2-20326]

Western Blot: SF3B4 Antibody [NBP2-20326]

Western Blot: SF3B4 Antibody [NBP2-20326] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: Jurkat 10% SDS PAGE gel, diluted at 1:1000.
SF3B4 Antibody

Western Blot: SF3B4 Antibody [NBP2-20326] -

Western Blot: SF3B4 Antibody [NBP2-20326] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SF3B4 antibody (NBP2-20326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SF3B4 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SF3B4

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq]

Alternate Names

Hsh49, pre-mRNA splicing factor SF3b 49 kDa subunit, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49MGC10828, SF3B49, SF3b50, spliceosomal protein, spliceosome-associated protein (U2 snRNP), Spliceosome-associated protein 49, splicing factor 3B subunit 4, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa

Gene Symbol

SF3B4

UniProt

Q15427 (Human)

Additional SF3B4 Products

Product Documents for SF3B4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SF3B4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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