SFPQ Antibody (6D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00006421-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

SFPQ (NP_005057, 269 a.a. ~ 361 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQ

Localization

Nuclear

Specificity

SFPQ - splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SFPQ Antibody (6D7)

Western Blot: SFPQ Antibody (6D7) [H00006421-M02]

Western Blot: SFPQ Antibody (6D7) [H00006421-M02] - SFPQ monoclonal antibody (M02), clone 6D7 Analysis of SFPQ expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: SFPQ Antibody (6D7) [H00006421-M02]

Immunocytochemistry/Immunofluorescence: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SFPQ Antibody (6D7) [H00006421-M02]

Immunohistochemistry-Paraffin: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on formalin-fixed paraffin-embedded human thyroid nodular goiter. Antibody concentration 3 ug/ml.

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Applications for SFPQ Antibody (6D7)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SFPQ

Essential pre-mRNA splicing factor required early in spliceosome formation. Binds to the mammalian polypyrimidine tracts. Forms a complex with the polypyrimidine tract-binding protein (PTB). Seems to also bind DNA. It is a heterotetramer of two 52 kDa and two 100 kDa subunits.

Alternate Names

100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, hPOMp100, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, Polypyrimidine tract-binding protein-associated-splicing factor, PSFPOMP100, PTB-associated splicing factor, PTB-associated-splicing factor, splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated), splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated), splicing factor proline/glutamine-rich, splicing factor, proline- and glutamine-rich

Entrez Gene IDs

6421 (Human)

Gene Symbol

SFPQ

OMIM

605199 (Human)

UniProt

NP_005057 (Human)

Additional SFPQ Products

Product Documents for SFPQ Antibody (6D7)

Product Datasheets

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COA

SDS

Product Specific Notices for SFPQ Antibody (6D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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