sFRP-1 Antibody (5I6V1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16851

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16851-100ul
NBP3-16851-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5I6V1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all sFRP-1 Antibodies »

Product Summary for sFRP-1 Antibody (5I6V1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human sFRP-1 (Q8N474). MGIGRSEGGRRGAALGVLLALGAALLAVGSASEYDYVSFQSDIGPYQSGRFYTKPPQCVDIPADLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for sFRP-1 Antibody (5I6V1)

Western Blot: sFRP-1 Antibody (5I6V1) [NBP3-16851]

Western Blot: sFRP-1 Antibody (5I6V1) [NBP3-16851]

Western Blot: sFRP-1 Antibody (5I6V1) [NBP3-16851] - Western blot analysis of extracts of various cell lines, using sFRP-1 Rabbit mAb (NBP3-16851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for sFRP-1 Antibody (5I6V1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-1

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Long Name

Secreted Frizzled Related Protein 1

Alternate Names

FrzA, SARP-2, sFRP1

Gene Symbol

SFRP1

Additional sFRP-1 Products

Product Documents for sFRP-1 Antibody (5I6V1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for sFRP-1 Antibody (5I6V1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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