sFRP-4 Antibody (7Z6Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16352

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16352-100ul
NBP3-16352-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Z6Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all sFRP-4 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-300 of human sFRP-4 (Q6FHJ7). AKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for sFRP-4 Antibody (7Z6Q1)

Western Blot: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Western Blot: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Western Blot: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Western blot analysis of extracts of various cell lines, using sFRP-4 Rabbit mAb (NBP3-16352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]

Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunohistochemistry of paraffin-embedded human colon using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for sFRP-4 Antibody (7Z6Q1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-4

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Gene Symbol

SFRP4

Additional sFRP-4 Products

Product Documents for sFRP-4 Antibody (7Z6Q1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for sFRP-4 Antibody (7Z6Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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