Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SFRS4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Pre-mRNA-Splicing Factor SRP75 using the numbering given in entry NP_005617.2 (Gene ID 6429).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFRS4 Antibody

Western Blot: SFRS4 Antibody [NBP2-04144]

Western Blot: SFRS4 Antibody [NBP2-04144]

Western Blot: SFRS4 Antibody [NBP2-04144] - J-Lat cells treated with sh vectors targeting SR proteins were induced with Prostratin over 24 hours. Cells were harvested for protein using RIPA. Blots for SRSF4, GFP, HIV p24 GAG protein, and the stain free gel are shown. The J-LAt cell line used had an inducibe HIV construct that when induced would produce GFP as well as HIV proteins such as p24 (GAG). The sh vector was used to knock down the protein. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144]

Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144]

Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144] - Sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1000 (1 ug/mL). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: SFRS4 Antibody [NBP2-04144]

Western Blot: SFRS4 Antibody [NBP2-04144]

Western Blot: SFRS4 Antibody [NBP2-04144] - CEM-T4 cell lysate. Western blot image submitted by a verified customer review.
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Applications for SFRS4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-10 ug/ml lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. SFRS4 antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-04144 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SFRS4

SFRS4 - splicing factor, arginine/serine-rich 4

Alternate Names

Pre-mRNA-splicing factor SRP75, serine/arginine-rich splicing factor 4, SFRS4, Splicing factor, arginine/serine-rich 4SRP001LB, SRP75SR splicing factor 4

Gene Symbol

SRSF4

UniProt

NP_005617.2 (Human)

Additional SFRS4 Products

Product Documents for SFRS4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SFRS4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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