SGK1 Antibody (4D7-G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00006446-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D7-G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SGK1 Antibodies »

Product Specifications

Immunogen

SGK (AAH01263.1, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL

Localization

Cytoplasmic and Nuclear (on phosphorylation).

Specificity

SGK - serum/glucocorticoid regulated kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SGK1 Antibody (4D7-G3)

Western Blot: SGK1 Antibody (4D7-G3) [H00006446-M01]

Western Blot: SGK1 Antibody (4D7-G3) [H00006446-M01]

Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SGK1 Antibody (4D7-G3) [H00006446-M01]

Immunocytochemistry/ Immunofluorescence: SGK1 Antibody (4D7-G3) [H00006446-M01]

Immunocytochemistry/Immunofluorescence: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SGK1 Antibody (4D7-G3) [H00006446-M01]

Immunohistochemistry-Paraffin: SGK1 Antibody (4D7-G3) [H00006446-M01]

Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. Antibody concentration 5 ug/ml.
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Applications for SGK1 Antibody (4D7-G3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recominant protein for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SGK1

This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.

Long Name

Serum/Glucocorticoid Regulated Kinase 1

Alternate Names

SGK

Entrez Gene IDs

6446 (Human)

Gene Symbol

SGK1

OMIM

602958 (Human)

UniProt

AAH01263 (Human)

Additional SGK1 Products

Product Documents for SGK1 Antibody (4D7-G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SGK1 Antibody (4D7-G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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