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SH2D1B Antibody (2B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00117157-M04

Novus Biologicals, part of Bio-Techne
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H00117157-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SH2D1B (NP_444512.2, 54 a.a. ~ 132 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RIFREKHGYYRIQTAEGSPKQVFPSLKELISKFEKPNQGMVVHLLKPIKRTSPSLRWRGLKLELETFVNSNSDYVDVLP

Specificity

Reacts with SH2 domain containing 1B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SH2D1B Antibody (2B4)

Sandwich ELISA: SH2D1B Antibody (2B4) [H00117157-M04] - Detection limit for recombinant GST tagged SH2D1B is 0.3 ng/ml as a capture antibody.
Immunoprecipitation: SH2D1B Antibody (2B4) [H00117157-M04]

Immunoprecipitation: SH2D1B Antibody (2B4) [H00117157-M04]

Immunoprecipitation: SH2D1B Antibody (2B4) [H00117157-M04] - Analysis of SH2D1B transfected lysate using anti-SH2D1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SH2D1B rabbit polyclonal antibody.

Applications for SH2D1B Antibody (2B4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SH2D1B

By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM]

Alternate Names

EAT2EAT-2, EWS/FLI1-activated transcript 2, SH2 domain containing 1B, SH2 domain-containing molecule EAT2, SH2 domain-containing protein 1B

Entrez Gene IDs

117157 (Human)

Gene Symbol

SH2D1B

Additional SH2D1B Products

Product Documents for SH2D1B Antibody (2B4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SH2D1B Antibody (2B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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