SH3BP2 [p Ser427] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-492
![Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]](https://resources.bio-techne.com/images/products/SH3BP2-%5Bp-Ser427%5D-Antibody-Western-Blot-NB600-492-img0003.jpg "Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SH3BP2 [p Ser427] Antibody
Immunogen
This affinity purified SH3BP2 [p Ser427] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP2 protein (SH3 Domain Binding Protein 2). (Uniprot: P78314)
Reactivity Notes
This product is phospho specific for human SH3BP2.
Modification
p Ser427
Specificity
This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human SH3BP2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human pS427.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SH3BP2 [p Ser427] Antibody
![Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]](https://resources.bio-techne.com/images/products/SH3BP2-[p-Ser427]-Antibody-Western-Blot-NB600-492-img0003.jpg "Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]")
Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]
Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492] - Detection of endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of 60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.![SH3BP2 [p Ser427] Antibody](https://resources.bio-techne.com/images/products/nb600-492_rabbit-polyclonal-sh3bp2-p-ser427-antibody-245202310564616.jpg "SH3BP2 [p Ser427] Antibody")
SH3BP2 [p Ser427] Antibody
Western blot analysis is shown using Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.Applications for SH3BP2 [p Ser427] Antibody
Application
Recommended Usage
ELISA
1:2000-1:10000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SH3BP2
Alternate Names
Abl-SH3 binding protein 2, Cherubism, CRBM, CRPM, FLJ42079, FLJ54978,3BP-2,3BP2, RES4-23, SH3 domain-binding protein 2, SH3-domain binding protein 2, TNFAIP3 interacting protein 2
Gene Symbol
SH3BP2
UniProt
Additional SH3BP2 Products
Product Documents for SH3BP2 [p Ser427] Antibody
Product Specific Notices for SH3BP2 [p Ser427] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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