Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N22/21

Concentration

1 mg/ml

View all SHANK1 Antibodies »

Product Summary for SHANK1 Antibody (N22/21)

Immunogen

Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1

Localization

Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density

Specificity

Detects approx 190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SHANK1 Antibody (N22/21)

Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915]

Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915]

Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunocytochemistry/Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.
Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).

Applications for SHANK1 Antibody (N22/21)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

0.1-1.0 ug/ml

Western Blot

1:1000
Application Notes
1 ug/ml of SHANK1 Antibody was sufficient for detection of Shank1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK1

1 ug/mL was sufficient for detection of Shank1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

SH3 and multiple ankyrin repeat domains 1, SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIPSPANK-1, synamon

Gene Symbol

SHANK1

Additional SHANK1 Products

Product Documents for SHANK1 Antibody (N22/21)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHANK1 Antibody (N22/21)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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