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SHANK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13215

Novus Biologicals, part of Bio-Techne
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NBP3-13215

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK2. The exact sequence is proprietary.

Reactivity Notes

Human (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

159 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SHANK2 Antibody

Western Blot: SHANK2 Antibody [NBP3-13215]

Western Blot: SHANK2 Antibody [NBP3-13215]

Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody [NBP3-13215]

Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody [NBP3-13215]

Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 antibody (NBP3-13215) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215]

Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215]

Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SHANK2 antibody (NBP3-13215) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SHANK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK2

SHANK2 - SH3 and multiple ankyrin repeat domains 2

Long Name

SH3 and Multiple Ankyrin Repeat Domains Protein 2

Alternate Names

AUTS17, CORTBP1, CTTNBP1, ProSAP1, SPANK-3

Gene Symbol

SHANK2

Additional SHANK2 Products

Product Documents for SHANK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SHANK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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