SHANK2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13215
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SHANK2 Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK2. The exact sequence is proprietary.
Reactivity Notes
Human (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
159 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for SHANK2 Antibody
![Western Blot: SHANK2 Antibody [NBP3-13215]](https://resources.bio-techne.com/images/products/SHANK2-Antibody-Western-Blot-NBP3-13215-img0001.jpg "Western Blot: SHANK2 Antibody [NBP3-13215]")
Western Blot: SHANK2 Antibody [NBP3-13215]
Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody [NBP3-13215]](https://resources.bio-techne.com/images/products/SHANK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13215-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody [NBP3-13215]")
Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody [NBP3-13215]
Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 antibody (NBP3-13215) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215]](https://resources.bio-techne.com/images/products/SHANK2-Antibody-Immunohistochemistry-Paraffin-NBP3-13215-img0002.jpg "Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215]")
Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215]
Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SHANK2 antibody (NBP3-13215) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for SHANK2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SHANK2
Long Name
SH3 and Multiple Ankyrin Repeat Domains Protein 2
Alternate Names
AUTS17, CORTBP1, CTTNBP1, ProSAP1, SPANK-3
Gene Symbol
SHANK2
Additional SHANK2 Products
Product Specific Notices for SHANK2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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