Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray

Label

Janelia Fluor 646

Antibody Source

Monoclonal Mouse IgG2B Clone # S69

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHANK3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 840-857 of rat Shank3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density

Specificity

Detects approx 190kDa. No cross-reactivity against Shank1 or Shank2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for SHANK3 Antibody (S69) [Janelia Fluor® 646]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Microarray

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SHANK3

The SHANK3 gene encodes a SH3 and multiple ankyrin repeat domain protein 3 that in isoform 1 is 1,741 amino acids long at 186 kDA and in isoform 2 is 1,732 amino acids long at 185 kDA. This protein functions as an adaptor in postsynaptic density of excitatory synapses. Through complexes that include GKAP/PSD-95 and Homer and the actin-based cytoskeleton, these excitatory synapses are connected with the postsynaptic membrane including NMDA-type and metabotropic glutamate receptor. Additionally, this protein may function in structural and organizational projects of the dendritic spine and the synaptic junction. SHANK3 gene participates in cytoplasmic microtubules, glutamatergic synapses, and signaling events regulated by ret tyrosine kinase. It is known to interact with genes PLCG1, FYN, SRC, ABL1, and PIK3R1. Defects in SHANK3 are associated with autism spectrum disorders and schizophrenia type 15. It is also associated with phelan-mcdermid syndrome, cerebritis, prostatitis, neuronitis, dementia, prostate cancer, hypogonadism, hepatitis a, jacobsen syndrome, and trisomy 22.

Alternate Names

DEL22q13.3, KIAA1650proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, PROSAP2, PSAP2SH3 and multiple ankyrin repeat domains protein 3, SH3 and multiple ankyrin repeat domains 3, shank postsynaptic density protein, Shank3, SPANK-2

Gene Symbol

SHANK3

Additional SHANK3 Products

Product Documents for SHANK3 Antibody (S69) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SHANK3 Antibody (S69) [Janelia Fluor® 646]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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