SHARPIN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27186

Novus Biologicals, part of Bio-Techne
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NBP2-27186
NBP2-27186SS
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Key Product Details

Species Reactivity

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SHARPIN Antibodies »

Product Specifications

Immunogen

A portion of amino acids 25-75 of human Sharpin was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHARPIN Antibody

Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186]

Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186]

Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186] - Formalin-fixed, paraffin-embedded human brain stained with Sharpin antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Applications for SHARPIN Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHARPIN

Sharpin (SHANk-associated RH domain interacting protein) is a 387-amino acid protein, enriched in the postsynaptic density (PSD) of excitatory neurotransmitters. It is abundantly expressed in brain, spleen, lung, heart, skeletal muscle, kidney and testis (Stieglitz, 2012). A component of the large multi-protein E3 ubiquitin ligase complex called LUBAC, Sharpin regulates cell survival and apoptosis, cytokine production, and development of lymphoid tissues by regulating TNF-alpha induced canonical NF-kappaB signaling. Sharpin binds to the LUBAC subunit HOIP, and stimulates the formation of linear ubiquitin chains in vitro and in vivo. Sharpin harbors multiple functional motifs including an amino terminal coiled-coil (CC) domain, which has been shown to mediate the interaction between Sharpin and the scaffold protein Shank. The other two domains, UBL and NPL4 zinc finger domain facilitate ubiquitin-mediated protein recognition and degradation. Sharpin also acts as an inhibitor of beta1-integrins activation and down-regulates the activity of tumor suppressor protein PTEN, thus modulating immune and inflammatory diseases (Wang, 2012).Sharpin antibody recognizes endogenous levels of total Sharpin, which is recently identified as a commonly up-regulated gene in many human cancers and is believed to have a role in tumor biogenesis. It may function in small GTPase-mediated signaling pathways that may transform the actin cytoskeleton, thereby inducing morphological changes needed for tumor metastasis (Jung, 2010). Thus, Sharpin antibody plays an important role in normal immune development and control of inflammation.

Long Name

SHANK-associated RH Domain Interactor

Alternate Names

SIPL1

Gene Symbol

SHARPIN

Additional SHARPIN Products

Product Documents for SHARPIN Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SHARPIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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