Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SHBG/245

Concentration

0.2 mg/ml

View all SHBG Antibodies »

Product Summary for SHBG Antibody (SHBG/245)

Immunogen

Recombinant full-length human SHBG protein (Uniprot: P04278)

Localization

Cytoplasmic & Extracellular (Secreted)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-48007)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SHBG Antibody (SHBG/245)

Immunohistochemistry-Paraffin: SHBG Antibody (SHBG/245) [NBP2-44993]

Immunohistochemistry-Paraffin: SHBG Antibody (SHBG/245) [NBP2-44993]

Immunohistochemistry-Paraffin: SHBG Antibody (SHBG/245) [NBP2-44993] - Human Testicular Carcinoma stained with SHBG Monoclonal Antibody (SHBG/245)

Applications for SHBG Antibody (SHBG/245)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SHBG

The gene encodes an EF hand-containing small calcium binding protein. It is an integral subunit component of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Kv4 potassium channels regulate action potentials in neurons and cardiac myocytes. The coexpression of ABP with pore-forming Kv4 alpha subunits causes changes in the gating and amplitude of Kv4 currents, thus profoundly affect the intracellular trafficking and molecular properties of Kv4.2 alpha subunits. It also cause a dramatic redistribution of Kv4.2, releasing intrinsic endoplasmic reticulum retention and allowing for trafficking to the cell surface. It may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. It maps to 5q35.1 region of human chromosome. Alternative splicing results in multiple transcript variant encoding different isoforms. Isoform 1 and isoform 2 are expressed in brain and kidney.

Long Name

Sex Hormone-binding Globulin

Alternate Names

ABP

Gene Symbol

SHBG

UniProt

P04278

Additional SHBG Products

Product Documents for SHBG Antibody (SHBG/245)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SHBG Antibody (SHBG/245)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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