SHC1 Antibody (6E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89051

Novus Biologicals, part of Bio-Techne
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NBP2-89051
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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E10

Concentration

LYOPH mg/ml

View all SHC1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of SHC1 phosphorylated at Ser36 conjugated to KLH.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Thiophilic adsorption and size exclusion chromatography purified.

Applications for SHC1 Antibody (6E10)

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Western Blot: 1 ug/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (6E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SHC1 Antibody (6E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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