SHC1 Antibody (6E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89051

Novus Biologicals, part of Bio-Techne
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NBP2-89051
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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E10

Format

BSA Free

Concentration

LYOPH mg/ml

View all SHC1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of SHC1 phosphorylated at Ser36 conjugated to KLH.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Thiophilic adsorption and size exclusion chromatography purified.

Applications for SHC1 Antibody (6E10) - BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Western Blot: 1 ug/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Formulation

Lyophilized from 1ml of 2x PBS, PEG, and sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (6E10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SHC1 Antibody (6E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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