Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A1

Concentration

1 mg/ml

View all SHC1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHC1 Antibody (OTI3A1)

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434]

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434]

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.
Immunocytochemistry/ Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunocytochemistry/ Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunocytochemistry/Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1 .
Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded prostate tissue using anti-SHC1 mouse monoclonal antibody.
View 8 more images

Applications for SHC1 Antibody (OTI3A1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Entrez Gene IDs

6464 (Human)

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (OTI3A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SHC1 Antibody (OTI3A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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