SHIP Antibody (9G11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26196
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9G11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human SHIP [UniProt Q92835]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHIP Antibody (9G11)
Immunoprecipitation: SHIP Antibody (9G11) [NBP3-26196] -
Immunoprecipitation: SHIP Antibody (9G11) [NBP3-26196] - Immunoprecipitating SHIP in Raji whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26196 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26196 (2ug) + Raji whole cell lysate(500ug).
Lane 3: Raji whole cell lysate (10ug).
Immunohistochemistry: SHIP Antibody (9G11) [NBP3-26196] -
Immunohistochemistry: SHIP Antibody (9G11) [NBP3-26196] - Image of SHIP Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SHIP Antibody (9G11) [NBP3-26196] -
Western Blot: SHIP Antibody (9G11) [NBP3-26196] - Positive Western Blot detected in: Raji whole cell lysate, K562 whole cell lysate.All lanes: SHIP Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 134, 110 kDa
Observed band size: 145 kDa
Applications for SHIP Antibody (9G11)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SHIP
Long Name
SH2-containing Inositol Phosphatase
Alternate Names
INPP5D
Gene Symbol
INPP5D
Additional SHIP Products
Product Documents for SHIP Antibody (9G11)
Product Specific Notices for SHIP Antibody (9G11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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