SHIP Antibody (SHIP-01) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-81538
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # SHIP-01
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Specificity
The SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SHIP Antibody (SHIP-01) - BSA Free
Western Blot Shows Human SHIP Specificity Using Knockout Cell Line.
Western blot shows lysates of iPSC-derived microglia and SHIP knockout iPSC-derived microglia (KO). Nitrocellulose membrane was probed with SHIP Antibody (SHIP-01) - BSA Free (Catalog # NB110-81538) followed by HRP-conjugated goat anti-mouse IgG Secondary Antibody. A specific band was detected for SHIP at approximately 135 kDa (as indicated) in the parental iPSC-derived microglia cell line, but is not detectable in knockout iPSC-derived microglia cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.![Western Blot: SHIP Antibody (SHIP-01)BSA Free [NB110-81538]](https://resources.bio-techne.com/images/products/SHIP-Antibody-SHIP-01-Western-Blot-NB110-81538-img0001.jpg "Western Blot: SHIP Antibody (SHIP-01)BSA Free [NB110-81538]")
Western Blot: SHIP Antibody (SHIP-01)BSA Free [NB110-81538]
Western Blot: SHIP Antibody (SHIP-01) [NB110-81538] - Analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02).Applications for SHIP Antibody (SHIP-01) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
2-5 ug/ml
Western Blot
1:1000
Application Notes
Western Blotting - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note: Reducing conditions. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SHIP
Long Name
SH2-containing Inositol Phosphatase
Alternate Names
INPP5D
Gene Symbol
INPP5D
UniProt
Additional SHIP Products
Product Documents for SHIP Antibody (SHIP-01) - BSA Free
Product Specific Notices for SHIP Antibody (SHIP-01) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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