SHMT1 Antibody (4F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006470-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 4F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

SHMT1 (NP_004160, 374 a.a. ~ 482 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPD

Specificity

SHMT1 - serine hydroxymethyltransferase 1 (soluble)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SHMT1 Antibody (4F9)

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01]

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - SHMT1 monoclonal antibody (M01), clone 4F9 Analysis of SHMT1 expression in HeLa.

Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01]

Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 1 ug/ml.

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01]

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody (M01), clone 4F9.Lane 1: SHMT1 transfected lysate(53.1 KDa).Lane 2: Non-transfected lysate.

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Applications for SHMT1 Antibody (4F9)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHMT1

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Long Name

Serine hydroxymethyltransferase, cytosolic

Alternate Names

EC 2.1.2.1, Serine methylase, SHMT

Entrez Gene IDs

6470 (Human)

Gene Symbol

SHMT1

OMIM

182144 (Human)

UniProt

NP_004160 (Human)

Additional SHMT1 Products

Product Documents for SHMT1 Antibody (4F9)

Product Datasheets

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COA

SDS

Product Specific Notices for SHMT1 Antibody (4F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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