Shugoshin Antibody (3C11)
Novus Biologicals, part of Bio-Techne | Catalog # H00151648-M01
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
ELISA, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3C11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SGOL1 (AAH17867, 1 a.a. ~ 292 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALENEKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQIPLEETELPGQGESFQIEATPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKRALEVSPAKEAIFILYYVREFVSRFPDCRKCKLETHICLR
Reactivity Notes
Other species not tested.
Localization
Nuclear. Localizes to the centromere. BUB1 is required for centromeric localization.
Specificity
SGOL1 - shugoshin-like 1 (S. pombe)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Shugoshin Antibody (3C11)
![Western Blot: Shugoshin Antibody (3C11) [H00151648-M01]](https://resources.bio-techne.com/images/products/Shugoshin-Antibody-3C11-Western-Blot-H00151648-M01-img0002.jpg "Western Blot: Shugoshin Antibody (3C11) [H00151648-M01]")
Western Blot: Shugoshin Antibody (3C11) [H00151648-M01]
Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.![Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01]](https://resources.bio-techne.com/images/products/Shugoshin-Antibody-3C11-Immunoprecipitation-H00151648-M01-img0001.jpg "Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01]")
Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01]
Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 transfected lysate using anti-SGOL1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGOL1 MaxPab rabbit polyclonal antibody.Applications for Shugoshin Antibody (3C11)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26670547).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Shugoshin
Alternate Names
FLJ14230, hSgo1, NY-BR-85, Serologically defined breast cancer antigen NY-BR-85, SGO, SGO1, shugoshin 1AB protein, shugoshin 1CD protein, shugoshin 1EF protein, shugoshin 1GH protein, shugoshin 1KL protein, shugoshin-like 1, shugoshin-like 1 (S. pombe)
Entrez Gene IDs
151648 (Human)
Gene Symbol
SGO1
OMIM
609168 (Human)
UniProt
Additional Shugoshin Products
Product Documents for Shugoshin Antibody (3C11)
Product Specific Notices for Shugoshin Antibody (3C11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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