Hi, we are trying to find out if the antibody NB110-88113 (SIAH2 Antibody (24E6H3) 0.5ml Cancer Marker, Stem Cell Marker) will be useful for our application. We want to try to look at Siah (sina in Drosophila) in drosophila larval brains, more specifically in the Drosophila neural stem cells. As in the webpage it says that this antibody recognizes Drosophila Sina we would like to ask if this antibody was tested in Drosophila tissues or Drosophila Cells.

Name: SIAH2 Antibody (24E6H3)
Brand: Novus Biologicals
SKU: NB110-88113

Our SIAH2 antibody with catalogue number NB110-88113 is covered by our 100% guarantee for the detection of SIAH2 in samples from Drosophila by Western blotting, Flow Cytometry, Immunofluorescence, and Immunohistochemistry with both frozen and paraffin-embedded samples. For further information on our guarantee please see the following link: Quality Assurance. The immunogen used to generate this antibody was a synthetic peptide made to an N-terminal portion (within residues 1-100) of the drosophila SINA protein (Swiss-Prot# P21461). Although we show data from human samples on our webpage for this antibody, we unfortunately do not have any images from Drosophila samples that I can share with you at the present time.

SIAH2 Antibody (24E6H3)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-88113

Submit a review

Citations (20)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Drosophila, Mouse (Negative)

Cited:

Human, Mouse, Insect - Drosophila

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 24E6H3

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all SIAH2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the drosophila SINA protein (within residues 1-100). [Swiss-Prot# P21461]

Localization

Nuclear, Cytoplasmic

Specificity

This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for SIAH2 Antibody (24E6H3)

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in human colorectal adenocarcinoma using NB110-88113. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.

Immunocytochemistry/ Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunocytochemistry/Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in ovarian carcinoma (OVCR3) cells using NB110-88113.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Strong SIAH2 staining in all nuclei in this basal-like breast carcinoma. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113]

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113] - Human HepG2 cell line was stained with Mouse Anti- SIAH2 Monoclonal Antibody (Catalog# NB110-88113, orange), or Mouse IgG1 isotype control (Catalog# MAB002, blue) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog# FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog# FC005).

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Moderate to strong staining of SIAH2 in the nucleus of a small proportion of the cell in a high nuclear grade ductal carcinoma in situ with comedo necrosis. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Occasional weak to moderate SIAH2 (arrows) staining in a luminal type ductal carcinoma. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] -

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

View 4 more images

Applications for SIAH2 Antibody (24E6H3)

Application

Recommended Usage

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10

Immunohistochemistry

1:40-1:50

Immunohistochemistry-Frozen

1:40-1:50

Immunohistochemistry-Paraffin

1:40-1:50

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SIAH2

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Alternate Names

E3 ubiquitin-protein ligase SIAH2, EC 6.3.2, EC 6.3.2.-, hSiah2, seven in absentia (Drosophila) homolog 2, Seven in absentia homolog 2, seven in absentia homolog 2 (Drosophila), Siah-2

Entrez Gene IDs

6478 (Human); 20439 (Mouse)

Gene Symbol

SIAH2

Additional SIAH2 Products

Product Documents for SIAH2 Antibody (24E6H3)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for SIAH2 Antibody (24E6H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

SIAH2 Antibody (24E6H3)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-88113

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all SIAH2 Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Clonality

Host

Isotype

Scientific Data Images for SIAH2 Antibody (24E6H3)

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunocytochemistry/ Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] -

Applications for SIAH2 Antibody (24E6H3)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: SIAH2

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional SIAH2 Products

Product Documents for SIAH2 Antibody (24E6H3)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for SIAH2 Antibody (24E6H3)